Tissue Culture Method for Toxigenicity Testing of Corynebacterium diphtheriae

Tissue culture method for toxigenicity testing of Corynebacterium diphtheriae.

A method for toxigenicity testing of Corynebacterium diphtheriae in tissue cultures was developed. Results were obtained by comparing destruction of the monkey kidney or, preferably, rabbit kidney monolayer by 0.1 ml of the C. diphtheriae culture in Elek's broth containing 20% rabbit serum with the appearance after the addition of 0.2 ml of a mixture of the C. diphtheriae culture and diphtheria...

A modified toxigenicity test for Corynebacterium diphtheriae.

Determination of Corynebacterium diphtheriae toxigenicity by a colorimetric tissue culture assay.

Chinese hamster ovary (CHO) cell cultures in microtiter wells are sensitive to growth inhibition and killing by picogram quantities of diphtheria toxin. In the absence of biologically active toxin, the CHO cell culture produces sufficient acidic metabolites to change the phenol red pH indicator from pink to yellow within 56 h. In the presence of 10 pg of toxin per well, growth inhibition can be...

Rapid determination of Corynebacterium diphtheriae toxigenicity by counterimmunoelectrophoresis.

A method for detecting toxin production in Corynebacterium diphtheriae cultures by counterimmunoelectrophoresis is described. Precipitin lines were observed in toxigenic strains in 30 min.

A Staining Method for Corynebacterium diphtheriae.

The following method is recommended for the staining of Corynebacterium diphtheriae. It provides a good contrast between the body of the organism and the granules in it. Cover the fixed and dried film with Loeffler's methylene blue, leave for five minutes, wash with water, decolorize rapidly with 1/1000 sulfuric acid. The acid should not stay on the film for more than a few seconds, wash with w...